Journal: Materials Today Bio

Article Title: Ultrasound-triggered carrier-free nanoprodrugs activate cGAS-STING pathway to enhance tumor-targeting chemo-immunotherapy

doi: 10.1016/j.mtbio.2026.102858

Figure Lengend Snippet: The characterizations of in vitro sonodynamic effects and cGAS-STING pathway activation. 4T1 cells were inoculated and incubated with PBSN38-CUR for 24 h, followed by characterization of (A) cytotoxicity, (B - D) apoptosis, and DNA damage after US irradiation (frequency: 3 MHz, intensity: 1.5 W cm −2 , duty cycle: 50 %, duration time: 1 min). Scale bar: 100 μm. (E) Release behavior of cellular dsDNA after various treatments. (F) Expression of STING, TBK1, and IRF3 proteins and their corresponding phosphorylated proteins after different treatments. ( G ) Maturation characterization of DCs after various treatments. Data were expressed as mean ± standard deviation of biological replicates (n = 3). One-way analysis of variance (ANOVA) followed by the post-Tukey's multiple comparisons test was used to compare multiple groups.

Article Snippet: Picogreen dsDNA quantitation reagent was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. InVivoMAb anti-mouse PD-L1 (B7-H1) antibody was purchased from Bioxcell.

Techniques: In Vitro, Activation Assay, Incubation, Irradiation, Expressing, Standard Deviation

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Activation of Fn14 activates the cGAS‑STING pathway in fibroblasts. A , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h. Immunofluorescence and confocal microscopy show the colocalization of Picogreen (green) and Mitotracker (red) in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. B , D-G , The protein levels of cGAS, STING, p-TBK1 Ser172 , and p-IRF3 Ser396 were determined by Western blot ( n = 3). C , Immunofluorescence and confocal microscopy show the colocalization of STING (green) and GM130 (red) in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: An equal volume of Picogreen dsDNA Quantitation Reagent (Yeasen, Shanghai, China) working solution was added to each cell sample and incubated for 5 min in the dark at 37°C for DNA staining.

Techniques: Activation Assay, Immunofluorescence, Confocal Microscopy, Western Blot

Journal: Infection and Drug Resistance

Article Title: LILRB1 Modulates Neutrophil Migration, NETosis, and Inflammation in Drug-Resistant Pseudomonas aeruginosa -Associated Bronchiectasis

doi: 10.2147/IDR.S581113

Figure Lengend Snippet: Qualitative and quantitative analysis of neutrophil extracellular traps (NETs) formation in neutrophils among different groups. ( A ) Immunofluorescence staining observation of NETs in neutrophils from different groups. ( B ) Comparison of NETs-dsDNA concentration in neutrophils among different. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: NETs-dsDNA was quantified using the Quant-iT PicoGreen dsDNA Quantitation Kit (Yeasen, Shanghai, China).

Techniques: Immunofluorescence, Staining, Comparison, Concentration Assay